A bacteria that is a histidine auxotroph should be grown on a medium that

Question

a) contains histidine

b) lacks histidine

c)contains glucose

d) contains an antibiotic

e) none of the above

I used to know this answer, I'm torn between a and b, although I could still be wrong lol

Answer 1

The medium must contain histidine.  The bacteria can non synthesize histidine and it must therefore be taken up from the environment.  

Answer 2

A. contains Histindine.

see definition of 'Auxotroph'- inability to synthesize organic compound i.e. Histidine. Thus, it must be provided from external source; culture medium in this case.

Answer 3

a) contains histidine