Question:

An advantage of fluorescence microscopy over staining techniques is that:?

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A. fluorescence micsoscopy can be used with any light microscope

B. fluorescence micsoscopy can be used with live specimens

C. fluorescence micsoscopy can be used with an electron microscope

D. fluorescence micsoscopy is less expensive and easier to use

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  1. The answer is B . there are many stains that are called vital stains in that they may be used in living cells and cause little or no damage to normal operations . Fluorescence is much more sensitive than normal colorimetic evaluations ..and if chosen correctly, some fluorescence assays can detect 10^-15 M of certain cell cycle intermediates , etc . There are available on the market many flurophores that can be used to track ion or molecule movement  into and targets inside cells.


  2. B.!

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