Any one have any idea how to separate chlorophyll from other compound in a plant extract?

Question

i extract my plant with 95% ethanol using soxhlet extraction method. now i try to seperate the chlorophyll from my extract, is there any method that can let this to be done?

Answer 1

What about chromotography

either paper

or column

using cellulose

run it through with an increasing concentration of salts?

Answer 2

Dear  Kuong

Before soxlet extraction with methanol or ethanol you completely dry the plant sample in shade and in hot air oven (below 60 degree). Thus you can minimize the chrophyll problem. In another way you can extract with petroleum ether or hexane to get rid off impurtities problem. This is my practical experience