Brassica Rapa Plants?

Question

Can someone please tell me the gene that makes the Brassica Rapa Plant grow exceedingly fast.thankyou

Answer 1

It is the FLC gene in the Brassica rapa which causes the fast or slow growth. The number gene copies vary from variety to variety. More the no. more fast is the flowering.

The following links and articles give the details,

Cloning and sequence analysis of Brassica FLC genes:

Plants of the biennial B. rapa oilseed cultivar, Per, were grown in a growth chamber for 2 weeks under long-day (LD) conditions (16 hr light:8 hr dark) at 21°. Total RNA was extracted from leaves using the TRI reagent (Sigma, St. Louis) as directed by the manufacturer. First strand of cDNA was synthesized with the SuperScript II reverse transcriptase (Life Science Technology, Gaithersburg, MD) using the poly(dT)-M13 primer (5'-GTA AAA CGA CGG CCA GTC CCT TTT TTT TTT TTT T-3'). Synthesized first strands of cDNA were used as templates to amplify BrFLC cDNA by using the FLC44 primer (5'-CGG CTT AGA TCT CCG GCG ACT-3') and the poly(dT)-M13 primer. The PCR products were cloned into pGEM-Teasy vectors (Promega, Madison, WI) and sequences were analyzed. All cDNA corresponded to a single BrFLC gene.

To isolate additional genomic Brassica FLC genes, conserved primers were designed by aligning the BrFLC cDNA with A. thaliana FLC cDNA (AF116527; Fig 1A, exon 2 and exon 7 primers) and used for 35 cycles of PCR with genomic DNA from doubled haploid lines of B. rapa (IMB218) and B. oleracea (TO1000). PCR products were excised from the gel, purified using the GFX PCR DNA and gel band isolation kit (Amersham Biosciences, Piscataway, NJ), and cloned into pGem T-Vectors (Promega).