Firefly luciferase is the enzyme that allows fireflies to illuminate their abdomens. Because this light generation is an ATP-requiring reaction, firefly luciferase can be used to test for the presence of ATP. In this way, luciferase can test for the presence of life. The coupled reactions are ...
1) luciferin O2 <--> oxyluciferin light
2) ATP<--> AMP PPi
If the overall Delta G of the coupled reaction is -5.50 kJ/mol, what is the K of the first of the above reactions at 30 degrees C?
Recall that the Delta G for the hydrolysis of ATP to ADP is -32.5 kJ/mol, and for ATP to AMP it is -31.6 kJ/mol.
Thanks for all your help !!
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