Congo Red-binding substance?

Question

Dear Sir/Madam,

Could you mind telling me, what's Congo Red-binding substance.

I do hope to hear from you soon.

Sincerely yours,

Kristi

Answer 1

This stain is used for the visual detection of amyloid in muscle and nerve fresh frozen sections in patients who have amyloidosis. The dyeing of amyloid is by a mechanism similar to the direct textile dyeing of cotton. The linearity of the dye configuration permits hydrogen bonding of the azo and amine groups of the dye to similarly spaced carbohydrate hydroxyl radicals of the amyloid substance. The use of a staining solution containing high content of alcohol and free alkali releases native internal hydrogen bonding between adjacent polysaccharide chains and creates more potential sites for the binding of the dye.http://neuromuscular.wustl.edu/pathol/hi...

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