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Cre-lox recombination?

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Can someone please explain this...

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  1. Sounds like a constipation drug to me.


  2. Comstock is so right.

  3. Whoo, this question got posted to the wrong area. Anyway, cre-lox recombination is a system scientists use to control cutting and attaching pieces of DNA. The system has two parts: a protein called cre and a stretch of DNA with a particular sequence, known as lox P. The cre protein cuts DNA whenever it finds a lox sequence. The cre protein basically cuts the lox sequence in half, leaving one half that is sticky and ready to be reconnected. If there is another lox sequence nearby in the DNA, the protein will patch one cut lox sequence to another cut sequence. The result is that all the DNA in between lox sequences is cut out and the ends are joined. Scientists can use this to cut out sections of DNA, and also to add in new DNA in between lox sequences if it has the "sticky" half-sections of the lox sequence. Cre-lox gives scientists a way to delete and add genes in DNA.
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