Cryopreserve skin tissue, how is it done?

Question

When skin tissue is being cryopreserved, what is the protocall to prepare the sample for storage?

Answer 1

Cryopreserved in vitro cultured epithelial tissue and method

A process for preserving cultured epidermal sheets by incubating the sheets

at room temperature in media with a glycerol or dimethylsulfoxide

cryopreservant for a predetermined period of time and then freezing the

cultured sheets. The cooling gradient is characterized as having a lower

initial rate of cooling followed by a higher rate of cooling.