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Describe berthelots reaction in nitrogenous compund in urine?

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Describe berthelots reaction in nitrogenous compund in urine?

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  1. NH4+ reacts with alkaline hypochlorite in the presence of nitroprusside, and is complexed with alkaline phenol. Precipitation of magnesium and calcium is prevented by complexing with a strong citrate buffer. The reaction mixture is heated to 80 C to accelerate the reaction, and absorbance is measured at 660 nm.

    http://www1.whoi.edu/eqpac-docs/proto-13...

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