Describe steps needed to prepare 0.5L OF 1M Tris-HCl ph 8.0.?

Question

Describe steps needed to prepare 0.5L OF 1M Tris-HCl ph 8.0.?

Answer 1

it has a molar mass of 157.59 g/mol

since yu are making 0.5L OF 1M Tris-HCl , you need a half a mole:

weigh out 78.80 grams,

dissolve it into some water,

dilute it to 0.5 litres

it should have a pH = pKa of TRIS which is 8.06,

so add what ever HCl is needed dropwise to get it to as close to 8.O that you wish