Do u think the proteins will migrate from positive to negative pole or the reverse in SDS-PAGE?

Question

Do u think the proteins will migrate from positive to negative pole or the reverse in SDS-PAGE?

Answer 1

Bound Protein will migrate toward the positive pole.

Answer 2

SDS is a ANionic detergent.  This means that the sulfated detergent molecule is negatively charged.  When it binds in high stoichiometry to protein molecules, it confers a high negative charge density.  The detergent bound protein will thus migrate to the positive pole.

In a gel, the top part is configured to have the black (negative) pole and the wire for the bottom of the gel is red (positive).

Answer 3

In SDS PAGE the SDS coats the protein subunits with a net negative charge arising from the sodium salt binding by its hydrophobic tail to the protein surface while the sulfate end encompasses the negative charge.The subunit assumes a rather ros shaped appearance and being negative will migrate toward the positive pole in a manner inversely proportinal to its molecular size..So by plotting distance moved toward + pole Vs log MW a fairly linear "standard curve " for ascertaining the molecular weights of the separated subunits can be derived