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During DNA replication process, how is the replication fork organised and maintained in procaryotic cells.?

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Does this have any thing to do with origin of replication?

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  1. The origin of replication is where it starts.  The DNA strands are separated by helicase and replication occurs in opposite directions on each strand (by polymerase) which starts by reading the primer first and carrying on to the template DNA.  Strand separation causes supercoiling ahead of the replication forks which is relieved by topoisomerase.  Some of this is hard to explain, but google okazaki fragments to see how the lagging strand replicates.  The leading strand replicates in one long strand so it's not too complicated. When the two forks meet their are many proteins that stop replication and allow the two now completely replicated sets of DNA to become separate, each having one parent strand and one daughter strand.  There are some images if you google 'DNA replication in prokaryotes', it's kind of easier to understand with pictures.


  2. there are two replication forks.. and when they get tangled up in the end, an enzyme will melt the part that's tangled up..

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