Exactly HOW do restriction enzymes cut DNA?

Question

How does this process work?

Thanks!

Answer 1

simply the restriction enzyme moves along both the DNA strands in direction 5'-- 3' until it finds certain base sequence of 4-7 nucleotides then it cuts at the recognition site or near it

example:

the sequence (5'    G . AATTC   3') is recognized by acertain restriction enzyme ,

the dot represents the recognition site at which the enzyme cuts the DNA

Answer 2

Nearly all restriction enzymes recognize palindromic sites--that is, the sequence from 5' to 3' on each strand are the same. Most restriction enzymes are dimers of two identical subunits that fit on the DNA so that their twofold axis of symmetry is the same as that of the sequence. The enzyme moves along the DNA strand, and when it reaches the target sequence, the DNA is distorted and the strands are cut.

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