Explain 10 step in glycolysis with diagram?

Question

Explain 10 step in glycolysis with diagram?

Answer 1

Glycolysis step 1:

Glucose phosphorylation catalysed by Hexokinase:

α-D-Glucose + ATP -> α-D-Glucose-6-phosphate + ADP + H+

δGo = -16.7 kJ/mol  

  

Glycolysis step 2:

Isomerization of glucose-6-phosphate catalysed by Phosphoglucoisomerase:

α-D-Glucose-6-phosphate <=> D-Fructode-6-phosphate

δGo = +1.7 kJ/mol  

  

Glycolysis step 3:

Second phosphorylation catalysed by Phosphofruktokinase:

D-Fructode-6-phosphate + ATP -> D-Fructose-1,6-bisphosphate + ADP + H+

δGo = -14.2 kJ/mol  

  

Glycolysis step 4:

Cleavage to two Triose phosphates catalysed by Alsolase:

D-Fructose-1,6-bisphosphate <=> Dihydroxyacetone phosphate + D-glyceroaldehyde-3-phosphate

δGo = +23.9 kJ/mol  

  

Glycolysis step 5:

Isomerization of dihydroxyacetone phosphate catalysed by Triose phosphate isomerase:

Dihydroxyacetone phosphate <=> D-glyceroaldehyde-3-phosphate

δGo = +7.6 kJ/mol  

Glycolysis: Energy generation phase

  

Glycolysis step 6:

Generation of 1,3-Bisphosphoglycerate catalysed by Glyceraldehyde-3-phosphate dehydrogenase:

D-glyceroaldehyde-3-phosphate + NAD+ +Pi <=> 1,3-Bisphosphoglycerate + NADH + H+

δGo = +6.3 kJ/mol  

  

Glycolysis step 7:

Substrate-level phosphorylation, 3-Phosphoglycerate catalysed by Phosphoglycerate kinase:

1,3-Bisphosphoglycerate + ADP <=> 3-Phosphoglycerate + ATP

δGo = -18.8 kJ/mol  

  

Glycolysis step 8:

Phosphate transfer to 2-Phosphoglycerate catalysed by Phosphoglycerate mutase:

3-Phosphoglycerate <=> 2-Phosphoglycerate

δGo = +4.4 kJ/mol  

  

Glycolysis step 9:

Synthesis of Phosphoenolpyruvate catalysed by Enolase:

2-Phosphoglycerate <=> Phosphoenolpyruvate + H2O

δGo = +1.7 kJ/mol  

  

Glycolysis step 10:

Substrate-level phosphorylation. Pyruvate synthesis catalysed by Pyruvate kinase:

Phosphoenolpyruvate + H+ + ADP -> Pyruvate + ATP

δGo = -31.4 kJ/mol