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Explain how an autoclave works in terms of appropriate gas law?

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explain briefly

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  1. Autoclave and steriliser are interchangeable – the former is the term generally use in labs, while the latter is used in medical and pharmaceutical applications.

    An effective autoclave must contain dry saturated steam and this is achieved by removing the air from both the load and the chamber. This can be accomplished by:

    · Automatic purging – used with high mass, low load items, for example bottled media. Freesteaming further advances the method.

    · Vacuum system – a vacuum is achieved before the introduction of steam. This method is used for loads that contain a number of air pockets, such as wrapped instruments or fabrics.

    Once all the air is removed, pressure and temperature is increased until the pre-selected temperature is reached by:

    · Injecting steam into the chamber via an internal steam source

    · Using a direct steam source

    · Using a heater built-in to the autoclave

    With the direct steam method cycle times are faster.

    When the desired temperature and time have been achieved, the steam supply ceases.  


  2. an autoclave uses heat to sterilize and anprolene is a gas that sterilizes w/o melting plastic

  3. I don't know the name of the law, if there is one, but an autoclave replaces the air with steam, which is more effective at sterilizing, then the temperature is increased, since the space inside is sealed off the pressure also increases so any liquids inside do not boil.

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