Fluorescent stains for staph epi

Question

I am currently having a lot of trouble with fluorescently trying to stain Staph epi. I do not which one works best, and the ones I'm using, I am not sure how effective it is, (the live/dead Baclight Viability stain). I think it leaves some reside on the surface and I can not get a fairly accurate image, and I need to use some sort of staining method, to be able to distinguish between the bacteria and the rough surface I am using.

Answer 1

I read your statement and did not find the question you were going to ask.