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How Artificial inoculation of Plasmodiophora brassicae with brocolli at glasshouse could be successfull.?

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I am maintaining soil PH 6.15 and temperature 15 to 24C at this moment but not getting any good result, help me with more tips.

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  1. It takes 6 to 7 weeks from planting, how much time have you given it? Also, it is spelled broccoli.

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