How can these bacteria now produce human insulin?

Question

For example, bateria can now be used to manuracture human insuin. This is very important for my insuin-dependent diabetic patients.

Answer 1

I know that researchers take plasmids ( circular DNA fragments) , and engineer them with the human gene for insulin and an anitbiotic resistance gene by using restriction enzymes to cut and piece them together. The bacteria are then introduced to the plasmid and pick it up. Researchers then introduce all bacteria to an antibiotic. Only the ones that picked up the plasmid will survive because the plasmid contains the antibiotic resistance gene. Therefore, because it is part of the DNA, it will produce insulin. That is the overly simplified process, but that is how it works