How do determine the sensivivity of methylene blue dye in detecting DNA ?

Question

I am a student and I am doing a science project but i am quite stuck here. What are the steps or how do i do an experiment to determine the sensivivity of methylene blue dye in detecting DNA ? Thanks buds...really appreciate it. XD

Answer 1

Start with a DNA sample of known concentration and make a series of dilutions. Run each of these in a gel and then stain with methylene blue. Note which is the last well where you can still see the DNA band and use that to calculate the sensitivity of the dye.