How do i make an antibody/What is the procedure in creating antibodies?

Question

Every year in school, we have a year long project in Science called the Experiment Research Design (ERD). For this year, our group's approved topic is on Molecular Biology. Our objective is to make anti-bodies or research on the effects of anti-bodies on bacteria that are not easily dealt with ampicillin. Our group is going to make use of Toxoplasmosis as the controlled bacterium and we would be researching on Treponema Pallidum and Chlamydia Trachomatis as to their nature and we will try to work on them. We are currently located in the Philippines so if you have contacts of M. Biologists from here, please contact us. If you have recommended sources from the internet or books, please leave them here.

Answer 1

Depends what you want to make an antibody to.  Proteins would typically be mixed with an adjuvant and injected into a rabbit or other animal, preferably one whose sera had been pre-tested and found to not have background reactivity in the assays in which you plan to use your antibody.  Injections would be repeated every month or two, and small samples of blood would be used to test which animals were producing the best antisera, again, in the assay you'll be using later, ideally.  Smaller antigens would be conjugated to a large molecule, commonly KLH.  Antibodies to whole bacteria - outside my expertise, but I'm guessing you might not need an adjuvant.

In the U.S., we have antibodies made by companies.  The costs of animal housing combined with the costs of proving compliance with regulations about keeping the animals maximally comfortable during this procedure, usually involving anesthesia under the care of a veterinarian, have made it cheaper to let companies do the work.  They have to follow the same rules, so the animals are treated as well or better, and we just send them the protein.