How do you transform B. subtilis?

Question

I'm looking for a protocol for transforming Bacillus subtilis with plasmid DNA. I've tried several with no success, so tips on how to troubleshoot, fine tune standard proceedures apreciated too.

Answer 1

Well, here's one:  http://www.bio.net/bionet/mm/methods/200...

And here's a place where someone has volunteered to help:  http://www.microbes.info/forums/lofivers...