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How does reverse transcriptase work when they convert mRNA to cDNA?

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How does reverse transcriptase work when they convert mRNA to cDNA?

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  1. Not sure pete actually answered it.

    It uses its inbuilt  template and converts RNA to cDNA 5'--->3'.  IN chromosomes one strand with convert continuously the other will have to stop and start to keep 5'--->3' resulting in fragments.  

    http://www.nature.com/nature/journal/v45...


  2. Reverse transcriptase is used, as you might expect, to copy RNA into DNA. This task is integral to cloning complementary DNAs (cDNAs), which are DNA copies of mature messenger RNA. The technique is usually initiated by mixing short (12-18 base) polymers of thymidine (oligo dT) with messenger RNA such that they anneal to the RNA's polyadenylate tail. Reverse transcriptase is then added and uses the oligo dT as a primer to synthesize so-called first-strand cDNA.

    http://arbl.cvmbs.colostate.edu/hbooks/g...

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