How to choose the solvent system for running the column?

Question

i am performing column chromatography n plant extact. i want to know what is the creteria for deciding the solvent system for running the solvent

Answer 1

It depends on the polarity of substances you want to separate and the density and type of sorbent in your column.

A site with good hints bearing upon column chromatography choice of solvents incl.:

http://www.luknova.com/html/tech_support...