Question:

How to convert 100 microgram/ml to 50 ng/ml? I need to put in 10ml solution.

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How to convert 100 microgram/ml to 50 ng/ml? I need to put in 10ml solution.

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  1. 50 ng/ml = 50* 10^-3 microgram/ml = 0,05 microgram/ml

    ur stock is 100 microgram/ml

    C1 * V1 = C2 * V2

    V1 = C2 * V2/C1

    V1 =  0,05 * 10/100

    V1 = 0,005 ml


  2. use the dillution formula:

    C1V1 = C2V2

    (100e-6 g/ml)(V1) = (50e-9 g/ml)(10ml)

    V1 = 5e-3 millilitres

    your answer: use 5 ul , (aka 5 microlitres), of 100 microgram/ml solution to make 10 mls of a 50 ng/ml slution

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