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How to optimize micropropagation technique for commercial production of plantlets?

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i also want to know about protocols for germination, callus generation, rapid multiplication of plantlets and media preparation.

please help me. if you have any links or website..please let me know because i have a test about this topic on 20 April 2008...

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  1. For optimization of micropropagation technique you need to know how many plantlets are to be produced in how many days. Then you can back calculate the steps and optimize the number of initial explants, requirement of medium, glassware etc.

    Protocols for germination, callus generation, rapid multiplication of plantlets and media preparation etc are available on web. You may check.

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