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How to perform Western Blot technique to study the expression of protein Glucose Transport?

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How to perform Western Blot technique to study the expression of protein Glucose Transport?

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  1. Wikipedia actually gives a nice overview of Western blotting:

    http://en.wikipedia.org/wiki/Western_blo...

    Exactly what protein is it that you want to look at?  Whatever it is, you would need antibodies suitable for Western blotting raised against that protein, and then secondary antibodies conjugated to a fluorochrome or horseradish peroxidase so that you can visualize the protein on a blot.

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