How we measure the mycocardial enzymes in Blood?

Question

How we measure the mycocardial enzymes in Blood?

AND

What are its properties and amount in normal condition?

Answer 1

The enzymes are measured by blood tests.  they can be done at bedside or in the lab.  

The usual tests are Trop-I and a CKMB.  But also there is a Myoglobin that can be done.  Depends upon the Dr.  

I'm not exactly sure how to answer the properties.  The increase in circulating cardiac enzymes in the blood is a result of muscle damage to the heart.  The normal values are pretty much negligible (0.0-0.3)

Any increase in Trop-I is significant.  The CKMB does experience some crossover with CKMM (from the body) and CKBB (brain).  In a stroke the CKMB will elevate but the Trop-I won't.

Answer 2

Which enzymes? Note troponin is not an enzyme. Do you mean creatine kinase (CK)? CK is found in all muscle. Its role is to cleave phosphate groups off creatine phosphate to provide a source of chemical energy for the muscle.

An example of a typical method method would be:

1) CK in the sample converts creatine phosphate and ADP to creatine and ATP.

2) Hexokinase converts the ATP plus glucose to glucose-6-phosphate and ADP.

3)Glucose-6-phosphate dehydrogenase converts the glucose-6-phosphate plus NADP+ to 6-phosphogluconate plus NADPH + H+.

NADPH absorbs at 340nm - the increase in absorbance at 340nm is proportional to the amount of CK in the sample.

Answer 3

lab tests with blood -Troponin & CKMB