In dna amplification..?

Question

why are there more than 1 dna being amplified?

i tink it has got to do with many primer dimers..

can anyonie expand on this?

Answer 1

As noted above, you can minimize primer dimers with good primer design, but it happens.  Otherwise, it's a matter of getting temperatures and times just right, sometimes to minimize hybridization of your primers to and amplification of unwanted sequences with which they have a partial match/mismatch.

Answer 2

I'm not quite sure what you mean. Do you mean why do you add two primers to a sample to amplify a gene?

DNA is double stranded, so to amplify both strands of your gene you need two primers, one primer that reads left to right, and another that reads the other strand right to left. Once you're amplification is finished you should have equal copies of both strands for your gene.

Primer dimers occur when your primers amplify each other and not the target gene, but well designed primers shouldn't do this.