In which of the following actions does RNA polymerase differ from DNA polymerase?

Question

RNA polymerase uses RNA as a template, and DNA polymerase uses a DNA template.

RNA polymerase binds to single-stranded DNA, and DNA polymerase binds to double-stranded DNA.

RNA polymerase does not need to separate the two strands of DNA in order to synthesize an RNA copy, whereas DNA polymerase must unwind the double helix before it can replicate the DNA.

RNA polymerase is much more accurate than DNA polymerase.

RNA polymerase can initiate RNA synthesis, but DNA polymerase requires a primer to initiate DNA synthesis.

Answer 1

The last one is correct

A.  Wrong.  RNA Polymerase also uses DNA as a template

B.  Wrong.  DNA is never single stranded

C. Wrong. RNA Polymerase also must unwind the double helix (about 13 base pairs) to replicate it. (see 1st link)

D. Wrong.  RNA Polymerase is far less acurate (see 2nd link)

E. Correct.  RNA polymerase synthesizes RNA, but does not need a primer to initiate synthesis. DNA polymerase synthesizes DNA, but needs a primer in order to initiate synthesis. (Quote from 3rd link below)

Answer 2

RNA polymerase can initiate RNA synthesis, but DNA polymerase requires a primer to initiate DNA synthesis.