Isolation of plasmid dna by boiling method?

Question

from bacteria

Answer 1

This method is similar to alkaline lysis, except here hot temperatures are used to denature the plasmid and genomic DNA. The bacteria are placed in a boiling water bath which lyses the cells and denatures the DNA. The solution is cooled, causing the plasmid DNA to reanneal and remain in solution while the genomic DNA precipitates. The bacteria are spun in a centrifuge and the supernatant containing the plasmid DNA is removed. This technique appears to be older and used much less frequently than the alkaline lysis technique.

Answer 2

Depends on heat