List out the components needed for DNA synthesis.?

Question

Arthur Kornberg and colleagues was able to show that DNA synthesis can be done in vitro.

Answer 1

isnt it like proteins with the correct shape

who knows, i dont think i know what im talking about.

ahah just finished biology so whatever.

Answer 2

DNA template that contains the DNA region (target) to be amplified.

Two primers, which are complementary to the DNA regions at the 5' (five prime) or 3' (three prime) ends of the DNA region.

A DNA polymerase such as Taq polymerase or another DNA polymerase with a temperature optimum at around 70°C.

Deoxynucleoside triphosphates (dNTPs; also very commonly and erroneously called deoxynucleotide triphosphates), the building blocks from which the DNA polymerases synthesizes a new DNA strand.

Buffer solution, providing a suitable chemical environment for optimum activity and stability of the DNA polymerase.

Divalent cations, magnesium or manganese ions; generally Mg2+ is used, but Mn2+ can be utilized for PCR-mediated DNA mutagenesis, as higher Mn2+ concentration increases the error rate during DNA synthesis

Monovalent cation potassium ions.

Answer 3

Components of DNA - DNA is made of three types of compounds

1 — Phosphate groups which are negatively charged and give DNA molecules a negative charge.

2 — A 5 carbon sugar, deoxyribose (hence the name deoxyribonucleic acid). There are two sugars found in nucleic acids; ribose is found in RNA (ribonucleic acid) and deoxyribose in DNA. Deoxyribose refers to the fact that one of the hydroxyl (OH) groups is missing from this sugar.

3 — Nitrogen bases — Four different nitrogen bases are found in DNA. They are adenine (A), cytosine (C), guanine (G) and thymine (T). Their structures are shown below.