Question:

Please Classify The Parts of the Microscope.?

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please group them. if the part is Optical. Illuminating or Mechanical

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  1. Eye piece or occular-holds lenses to increase magnification.

    Coarse adjustment-focuses image under low power.

    Fine adjustment-Focuses image under high and low power.

    Revolving nose piese-moves objectives into viewing position.

    Objectives-holds lenses that magnify.

    Stage clips-holds slide in place

    Stage-supports slide

    Diaphragm-controls amount of light passing through specimen

    Mirror-reflects light source

    Base-supports microscope


  2. Starting from the top

    The ocular lens: Which is the bit you look through.

    The body tube: The long-ish bit some people hold whilst looking through the ocular lens.

    The revolving nose piece: Where the objectives are attached too

    The objectives: Attached to the revolving nose piece, and the bits that dermine the focus.

    The stage: Moves up and down, and is where you put what you are looking at on top of.

    Stage clips: Holds sample in place

    Course adjustment, and fine adjustment k***s: Adjust the focus.

    Light sourse: Usually a bulb

    Base: Explains itself really

  3. The compound  microscope has two systems of lenses for greater magnification, 1) the ocular, or eyepiece lens that one looks into and 2) the objective lens, or the lens closest to the object.  Before purchasing or using a microscope, it is important to know the functions of each part.  

    Eyepiece Lens:  the lens at the top that you look through.  They are usually 10X or 15X power.

    Tube:  Connects the eyepiece to the objective lenses

    Arm:  Supports the tube and connects it to the base

    Base:  The bottom of the microscope, used for support

    Illuminator:  A steady light source (110 volts) used in place of a mirror.  If your microscope has a mirror, it is used to reflect light from an external light source up through the bottom of the stage.

    Stage:  The flat platform where you place your slides.  Stage clips hold the slides in place.  If your microscope has a mechanical stage, you will be able to move the slide around by turning two k***s.  One moves it left and right, the other moves it up and down.

    Revolving Nosepiece or Turret:  This is the part that holds two or more objective lenses and can be rotated to easily change power.

    Objective Lenses:  Usually you will find 3 or 4 objective lenses on a microscope.  They almost always consist of 4X, 10X, 40X and 100X powers.  When coupled with a 10X (most common) eyepiece lens, we get total magnifications of 40X (4X times 10X), 100X , 400X and 1000X.  To have good resolution at 1000X, you will need a relatively sophisticated microscope with an Abbe condenser.  The shortest lens is the lowest power, the longest one is the lens with the greatest power.  Lenses are color coded and if built to DIN standards are interchangeable between microscopes.  The high power objective lenses are retractable (i.e. 40XR).  This means that if they hit a slide, the end of the lens will push in (spring loaded) thereby protecting the lens and the slide.  All quality microscopes have achromatic, parcentered, parfocal lenses.

    Rack Stop:  This is an adjustment that determines how close the objective lens can get to the slide.  It is set at the factory and keeps students from cranking the high power objective lens down into the slide and breaking things.  You would only need to adjust this if you were using very thin slides and you weren't able to focus on the specimen at high power. (Tip: If you are using thin slides and can't focus, rather than adjust the rack stop, place a clear glass slide under the original slide to raise it a bit higher)

    Condenser Lens:  The purpose of the condenser lens is to focus the light onto the specimen.  Condenser lenses are most useful at the highest powers (400X and above).  Microscopes with in stage condenser lenses render a sharper image than those with no lens (at 400X).  If your microscope has a maximum power of 400X, you will get the maximum benefit by using a condenser lenses rated at 0.65 NA or greater.  0.65 NA condenser lenses may be mounted in the stage and work quite well.  A big advantage to a stage mounted lens is that there is one less focusing item to deal with.  If you go to 1000X then you should have a focusable condenser lens with an N.A. of 1.25 or greater.  Most 1000X microscopes use 1.25 Abbe condenser lens systems.  The Abbe condenser lens can be moved up and down.  It is set very close to the slide at 1000X and moved further away at the lower powers.  

    Diaphragm or Iris:  Many microscopes have a rotating disk under the stage.  This diaphragm has different sized holes and is used to vary the intensity and size of the cone of light that is projected upward into the slide.  There is no set rule regarding which setting to use for a particular power.   Rather, the setting is a function of the transparency of the specimen, the degree of contrast you desire and the particular objective lens in use.

    How to Focus Your Microscope:  The proper way to focus a microscope is to start with the lowest power objective lens first and while looking from the side, crank the lens down as close to the specimen as possible without touching it.  Now, look through the eyepiece lens and focus upward only until the image is sharp.  If you can't get it in focus, repeat the process again.   Once the image is sharp with the low power lens, you should be able to simply click in the next power lens and do minor adjustments with the focus k**b.  If your microscope has a fine focus adjustment, turning it a bit should be all that's necessary.   Continue with subsequent objective lenses and fine focus each time.

    What to look for when purchasing a microscope.

    If you want a real microscope that provides sharp crisp images then stay away from the toy stores and the plastic instruments that claim to go up to 600X or more.  There are many high quality student grade microscopes on the market today.  They have a metal body and all glass lenses.  One of the most important considerations is to purchase your instrument from a reputable source.  Although a dealer may give you a great  price, they may not be around next year to help you with a problem.   One dealer that we can highly recommend is Microscope World.   They offer a wide variety of instruments at very competitive prices.  

    I hope that this helps hon

  4. since two already answered your question completely..i'll just add some facts.

    there are two types of microscopes

    one is the transmission electron microscope which is used to see the internal cell structure.the other one is the scanning electron microscope for the cell surface.

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