Question about protein methods?

Question

I have a bacteria that has on its membrane a protein(the protein is not a receptor).

I don't know the protein sequence and protein structure.

I also don't know which antibodies can binds with that protein.

How can i identify that protein using Western blot or Elisa.

Please write all that steps that should be done in order to identify that protein and explain them.

Thanks

Answer 1

1)  Purify protein

2)  Mix protein with adjuvant (complete Freunds, Incomplete Freund's or other of choice).  This helps you get better antibody titers.

3)  Inject protein into an animal - usually a goat, sheep or rabbit.

4)  Harvest serum and purify antibodies using affinity chromatography.

5)  Test antibodies out in Western blot format.