Reagent calculation.?

Question

the test required that you pipet 2 ml of serum into 8 ml reagent A. then take 1 ml of that solution and add it to 4 ml of solution B . What is the final dilution of the serum?

Answer 1

You take 2 mL and put it into 10 mL total volume, so it is a:

10 / 2 = 5 fold dilution.

You take 5 fold diluted solution and add 1 mL of it into 5 mL total volume. That gives you:

5 / (1/5) = 25 fold dilution.