Restriction site best for inserting a DNA fragment for analysis

Question

which restriction site is best for inserting a DNA fragment for analysis?

a) BamHI

b) EcoRI

c) HindIII

d) They're all equally good.

Answer 1

BamHI binds at the recognition sequence 5'-GGATCC-3'  , and cleaves these sequences just after the 5'-guanine on each strand. This cleavage results in "sticky ends" which are 4 b.p. long.

EcoRI recognizes the palindromic sequence GAATTC and cuts between the G and the A on both the top and bottom strands,producing sticky ends.

HindIII cleaves the palindromic DNA sequence AAGCTT and results in sticky ends

"d" is correct.

All are fine  - it depends on the DNA sequences of the gene and vector as to which are used.

Answer 2

They're all equally good.