Suppose you want 1mL of a 10microgram/mL solution?

Question

Suppose you want 1mL of a 10microgram/mL solution. How much water and protein stock must you add to get this?

Answer 1

what is the strength of your protein stock?

we will plug it into a dilution equation that tells you how much stock to use, the rest of the ml is water:

C1 V1 = C2 V2

(stock  strength) (V1) = (10microgram/mL) (1ml)

(V1) = (10microgram/mL) (1ml) / (stock  strength)