TACTGCTCCGAATCAGTCTAAACA ?

Question

Using the template DNA strand above provide the DNA sequence of the non-template DNA. Indicate the 5’ and 3’ ends of the molecule.

Answer 1

This shouldn't be hard for you. All you do is match up the nucleotides.

A pairs with T

G pairs with C

If you are given a C, then on the next strand you will have a G

No, either end cannot be the 3'.

You can tell because at the beginning of the stand you have TAC, which would be transcribed into AUG.

Thus AUG is your start codon in RNA. This is your starting end, thus your methylated end. The poly a tail is on the 3' end.

Since DNA is antiparallel, this will let you know if it is 3' or 5'.

I gave you all the info you need to now. Now, just figure out if it is 3' or 5'.

Answer 2

ATGACGAGGCTTAGTCATTTGT   AND  I THINK EITHER END CAN BE 3' OR 5'.