The polymerase chain reaction requires

Question

The polymerase chain reaction requires

a) primers complementary to the ends of the sequence to be amplified

b) careful temperature control

c) both of the above

d) neither of the above

Answer 1

You need primers so that the DNA polymerase has something to work from.  Otherwise, you'd get either random amplification or none at all.

Temperature is important in controlling denaturating, annealing and amplification.  It's an important variable in determining how much product you get.

Answer 2

C

Answer 3

It's both.

Answer 4

c) both of the above