The restriction enzyme Smal cuts DNA at the 6-base recognition sequence 5’-CCCGGG-3’.?

Question

Calculate the frequency of occurrence of this sequence

Answer 1

Sanjay V is almost right, but 4^6 is 4096 (i.e. once every 4 kb approximately).

However, this is an oversimplification and only works when A, C, G and T are present in equal amounts on each strand of the DNA.

A close approximation can be got by adjusting for the GC ratio (proportion of GC bases compared to AT).

Answer 2

SmaI is hexamer recognising enzyme its frequency is 4 is multiplied by 6 times it is equal to 16384

recognition site come after every 16384 base pairs