This is the sequence of a piece of mRNA.?

Question

write the amino acid sequence of the protein that would be formed by translating this piece of mRNA.

5' AUGGGCUUCCAGGACGGUCGG 3'

using the sequnce above, describe what would happen to the protein product of this piece of mRNA in the following 2 scenarios:

1. if a C were changed to a U:

what type of mutation is this?

2. If a C were deleted:

What type of mutation is this?

If a C were changed to a

Answer 1

5' AUG GGC UUC CAG GAC GGU CGG 3'

Would translate into:

Methionine-Glycine-Phenylalanine

-Glutamine-Aspartic Acid-Glycine

-Arginine

1. if a C were changed to a U:

Which C?  I'm going to assume the first one.

If the first "C" is changed to a U, the new codon GGU still codes for Glycine and the amino acid sequence is not changed.  In terms of DNA, it is a point mutation, but in terms of protein sequence it is referred to as a silent mutation.

2. If a C were deleted:

If the first C is deleted:

5' AUGGGCUUCCAGGACGGUCGG 3'

Methionine-Glycine-Serine

-Arginine-Threonine-Valine

This is an example of a frameshift mutation.

Notice that in this type of mutation, every amino acid after the change (in this case everything after Glycine) is also changed.

Answer 2

5'AUGGGCUUCCAGGACGGUCGG 3'

1. substitution

2.deletion

it would result in frameshift and alter the whole sequence from there on

Answer 3

Heh.. sometimes I wonder if those who ask questions here are even trying to figure out how to answer it on their own before they ask for help.

Starting from the 5' end to the 3' end, simply change each letter by the following formula:

A -> G

G -> A

C -> U

U -> C

Then look at the protein codon table in your class notes, your textbook, or on wiki, located here: http://en.wikipedia.org/wiki/Genetic_cod...

Every three letter code you make is a codon.  Start codons mean you can start producing proteins.  Stop codons means that you have to stop producing, no matter how much RNA is left (until you hit another start codon).

For what kind of mutation is what, look in your notes, your textbook, or wiki: http://en.wikipedia.org/wiki/Mutation#Ty...

There's a list of possible mutations, I'm sure you can figure them out.