What are the limitation of sudan iv in the lipid test?

Question

I need to know the answers now because i need to send the bio repot tomorrow. i don't even know what the h**l is the answer is because i'm just a kid. and one more question, why the sudan iv test is more sensitive towards the small quantities of lipid than using the paper test. if you give me the best answer i can make you gain an extra point XD

Answer 1

I don't know if there is any limitation except I know that Sudan III is more sensitive for lipid detection test. And Sudan IV is not soluble in water but in lipid.

_________________________________

Nextly, it can be used only to detect or test a saturated fat. For unsaturated fats it does work.

http://www.science.mcmaster.ca/Biology/f...

___________________________________

Thirdly, I knew that this agent is( Sudan IV stain or solution is restricted in wider commercial use and also it is a carcinogenic stain. (causing cancer)

But what disadvantages are there in lab for lipid testing I can hardly think of.

Here is a site with Sudan IV solution. May be you will notice some pros and cons of this solution...

http://en.wikipedia.org/wiki/Sudan_IV

COMPARE WITH  sudan III stain

http://en.wikipedia.org/wiki/Sudan_III

___________________________________

For staining purpose this solution is made up with Propylene glycol. AND it is perhaps very toxic  to health for longer use..

http://en.wikipedia.org/wiki/Propylene_g...