What equipment do you need in a cell culture lab's specimen preparation area?

Question

I'm designing a cell culture lab. What equipment do you need in the specimen preparation area?

Answer 1

u will need-

autoclave ,shaker,magnetic steerer,laminar flow,spirit lamps,microscopes (like sterio zoom microcopes),steam bath ,refrigerater ,weighing machine ,spectrophotometer ,centrifuge etc.

Answer 2

I am not sure what you meanby specimen preparation. If you are doing primary cell culture, i.e. taking tissues from a plant or animal, and placing them in culture to attempt to get the cells to grow then the requirements are different from the requirements of cell culture involving immortalized cell lines.

Also primary culture can vary in the necessities if you are culturing plants such as orchids or animal tissues such as fibroblasts.

Primary cell culture of animal tissues requires a dissection area (preferably inside a sterile hood). This area then would require dissection tools. possibly a dissecting microscope, warm waterbath etc.

Also sterlizing agents such as ethanol, cavacide, bleach, etc. As well as  the culture reagents needed to keep the cells alive once dissected including culture dishes, pipets, the appropriate media, with adatives such as antibiotics, calf serum, etc. (These will vary based on the type of cells you are culturing).

You will then need incubators for culturing the cells with regulated Co2 and moisture content.

For culture of cell lines some of the requirements may be similar but you are best to have separate (cell line only) hoods, incubators, reagents (media, additives etc) culture dishes etc. (again specific to the cell line). to avoid contamination.

You will also need autoclaves for sterilization, microscopes to see the cells, haemocytometers to count cells, centrifuges to spin down and pellet cells for splitting or expanding cultures, etc. and preferably liquid nitrogen storage for preserving cell lines over the longer term.

As you can see this is a very complicated and very expensive proposition, but I hope this helps.

Best to get a good book on cell culture techniques specific to the type of cells you are working with. Often you can get these free from the manufacurers of related reagents (Sigma, Clonetech, Fisher, Invitrogen, etc)