What is chemical method in haemoglobin estimation?

Question

What is chemical method in haemoglobin estimation?

Answer 1

The cyanohaemoglobin method -

Using a pipette add a sample of 20 µl of blood to 5ml of Drabkin’s solution in a test tube and mix thoroughly.  

DRABKIN’S SOLUTION:-

200 mg Potassium ferricyanide

50 mg  Potassium cyanide

140 mg  Potassium dihydrogen phosphate      

Distilled water made up to 1 Litre



Place approximately 2ml of the resulting solution into a cuvette, and read the absorbance values in a spectrophotometer at 540nm.

The haemoglobin concentration of the blood sample can be calculated from a curve prepared from known standards.

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