Question:

What is the difference between a spectrophotometer and flurometer?

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I am trying to make sense of a lab I completed and Im a bit confused on which was used to measure what and why.

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  1. The spectrophotometer is reading the amount of light the sample absorbs at a specific wavelength:

    1.  Generate light of a specific wavelength.

    2.  Shine light through sample.  Some of the light is absorbed by the sample.

    3.  Measure amount of light remaining of that wavelength.

    The fluorometer is reading the amount of light the sample generates at a specific wavelength when a different wavelength is shone on it:

    1.  Generate light of a specific wavelength.

    2.  Shine light through sample.  Some of the light is absorbed by the sample, and then released as a different wavelength.

    3.  Measure the amount of the different wavelength generated.

    Basically, the spectrophotometer sends in and measures the same wavelength.  The fluorometer sends in one wavelength, and then measures a different one coming out.

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