What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid i

Question

I. Transform bacteria with recombinant DNA molecule.

II. Cut the plasmid DNA using restriction enzymes.

III. Extract plasmid DNA from bacterial cells.

IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.

V. Use ligase to seal plasmid DNA to nonplasmid DNA.

a IV, V, I, II, III

b III, II, IV, V, I

c I, II, IV, III, V

d III, IV, V, I, II

e II, III, V, IV, I

Answer 1

b III, II, IV, V, I