What would be an ideal vector to use in agrobacterium to express a gene in a plant?

Question

What would be an ideal vector to use in agrobacterium to express a gene in a plant?

I am using pMDC 32 and pEarley Gate 100 in agrobacteria (C58C1) with a cDNA insert and injecting it into N. benthiana and arabadopsis. I had trouble with pmdc32 because I saw no response in my positive control. Wondering if anyone can suggest a plasmid to use that works well in agrobacteria, preferably one with gateway att sites.

Answer 1

hard question, not sure if i know enough to answer but...

did u try ti plasmid?

here's a list of other pdmcs

http://botserv1.uzh.ch/home/grossnik/cur...