Whats is the purpose of control plates (a)LB plate-pGLO and (b) LB/amp plate: pGLO??

Question

I did this in a practical involving bacterial transformation.

1st agar plate- LB/amp plate- +pGLO

2nd agar plate-LB/amp/ara plate +pGLO

3rd agar plate-LB/amp plate -pGLO

4th agar plate-LB plate -pGLO

To be frank with u..i was clueless the wholke practical..just hate genetics..thats all!

Answer 1

LB/Amp + pGLO = you will see transformants, but not expression of the gfp protein

LB/Amp/Ara = transformants grow AND the arabinose has activated the process for making the gfp protein... CELLS GLOW!

LB/AmP (-) pGLO = Control plate, you should not see any growth because pBAD has not been replaced in the E.coli cells (there is nothing to transform) so the E.coli will not grow on the antibiotic laced food. If there is growth here, then you will need to consider that a mistake was made somewhere (what does the growth look like?  a lawn or individual colonies?  Did you add pGLO instead of water? , was the ampicillin added to the plate?)


LB (-) pGLO = control plate, you will see growth here because you have E.coli cells that like growing on LB (its their food).