Which amycolatopsis orientalis strain produce L-epivancosamine sugar attached to chloroeremomycin?

Question

When I searched for epivancosamine sugar genes in NCBI, I found the sugar was produced in Amycolatopsis orientalis. But the same strain is written to produce vancomycin and chloroeremomycin separately. I am confused. Please help me to select the correct strain having nucleotide sequences deposited in NCBI. does sombody know how and where can I get the strain?

Answer 1

chloroeremomycin producing strain Amycolatopsis orientalis (A82846)

chloroeremomycin producing strain Amycolatopsis orientalis (A82846) contains three glycosyltransferases (Gtfs), GtfA, -B, and -C, along with the nonribosomal peptide synthetases genes, genes encoding nonproteinogenic amino acids, 4-OH-PheGly and 3,5-dihydroxyphenylglycine (3,5-(OH)2-PheGly), in the peptide backbone and genes for conversion of dTDP-d-glucose to dTDP-l-4-epi-vancosamine (11, 12). GtfB attaches the d-glucosyl moiety to 4-OH-PheGly4 of the vancomycin aglycone (AGV), generating the monoglucosylheptapeptide desvancosaminyl-vancomycin (DVV), and has a homologue GtfE in vancomycin biosynthesis (13). GtfC completes the disaccharide chain by using dTDP-l-4-epi-vancosamine and DVV to create epivancomycin. In the vancomycin biosynthesis pathway, the GtfC homologue, GtfD, transfers dTDP-l-vancosamine to DVV to produce vancomycin 1 (13). It has been proposed that GtfA, like GtfC, would use dTDP-l-4-epi-vancosamine as a sugar donor but show distinct regiospecificity by glycosylating the benzylic hydroxyl of the β-OH-Tyr6 residue to complete the biosynthetic pathway (12).

I hope I answered ur question.

give a try from ATCC , they have the micro organisms strains from all over the world.