Whichof the following can produce sticky ends in dna sequences?

Question

a. plasmids

b. transformation

c. restriction enzymes

d. bacteria

Answer 1

C. Restriction enzymes. However some enzymes produce blunt end, so not a good question.

Answer 2

Restriction enzymes - used for 'cutting' the DNA at certain base sequences and then combining it with other genetic material (where the 'sticky ends' connect).

This is especially used for recombinant DNA (for example, developing bacteria that can secrete human insulin).

Answer 3

Restriction enzymes can produce sticky ends (or blunt ends) by cutting DNA strands at a particular sequence. Everytime that sequence is found in a DNA strand the restriction enzyme cuts the DNA. Depending on the sequence the different types of ends are produced.