Why must you further dilute a sample if the absorbancy is 3 times the absorbancyof the protein standard is?

Question

we are determining the mg of protein in a sample by using a spectrometer.

Answer 1

You need to be careful not to saturate the spec.  Also, you don't want the concentration of an unknown sample to give a greater absorbance than the highest concentration of the standard you are using.  When you graph absorbance vs. concentration of the standard, your unkown needs to fall somewhere on that curve.